Development of a multiplex polymerase chain reaction (m-PCR) for the detection and identification of virulent and avirulent forms of Vibrio parahaemolyticus

This study developed a new multiplex polymerase chain reaction (m-PCR) for rapidly detecting clinically essential strains of V. parahaemolyticus. enables the detection total and potentially virulent strains. The m-PCR was by targeting species-specific transcriptional regulator toxR gene, sequences an outer membrane protein hypothetical encoded omp htp, respectively. htp were discovered originally randomly amplified polymorphic DNA (RAPD)-PCR. performed on parahaemolyticus isolates, 23 clinical 32 environmental. gene 367 bp fragment amplification found in all tested; 17 out isolates (73.91%) showed htp. Four sequence but not 2 exhibited omp. Therefore, both must be targeted PCR to detect Of other species tested, no seen. confirms that RAPD-PCR helps differentiate avirulent forms. allowed development identifying Keywords: Multiplex PCR, RAPD-PCR, Vibrio